What is ULTRON HF-Phenyl
- The ULTRON HF-Phenyl column is capillary column that employ monolithic silica (hierarchical porous structure) bonded with phenyl groups for high-precision separation analysis.
- Due to the π π interaction, the separation pattern is different from the other HF series.
- Enables separation proteins that have not been enzymatically digested.
- Enables suppression of carry-over by deactivation treatment on the silica surface.
- Enables analysis of proteins under low-pressure condition.
- It can be expected to searching unknown components and simultaneous analysis of various proteins.
- The column inner diameter is 0.1 mm and can be used for nano flow LC system.
- Suitable for LC/MS that requires high sensitivity detection.
- The column is protected by a PEEK column jacket, making it easy to handle.
ULTRON HF-Phenyl Specifications
| Column name | ULTRON HF-Phenyl |
|---|---|
| Column type | Phenyl |
| Column size (length x inner diameter) |
250 mm x 0.1 mm 700 mm x 0.1 mm |
| Applications | Peptides, Intact proteins |
| Flow range | Recommended range: 300〜500 nL/min |
| pH range | Recommended range: 2.0〜8.0 |
| Recommended temperature range | FEP sleeve: 30〜50°C (Max. temp. 50°C) PEEK sleeve: 30〜60°C (Max. temp. 60°C) |
| Max allowable pressure | FEP sleeve: 20.0 MPa PEEK sleeve: 30.0 MPa |
ULTRON HF-Phenyl Effect of column temperature (protein mixture)
In case the molecular weight is around 80 kDa, enables protein separation at temperatures close to body temperature.
ULTRON HF-Phenyl Column batch reproducibility (protein mixture)
Good reproducibility among column batches.
ULTRON HF-Phenyl Separation comparison with different column length (protein mixture)
Longer length columns achieve more defined separations.
ULTRON HF-Phenyl Effect of column temperature on mAb analysis
Enables analysis of mAb (145 kDa) at higher than 45℃.
ULTRON HF-Phenyl Carry-over of mAb
The column has showed more than 85% recovery at 50℃ column temperature.
ULTRON HF-Phenyl Analysis of positional isomers under water rich mobile phase
Due to π π interaction, the ULTRON HF-Phenyl column has ability to recognize positional isomers and different separation pattern from the ULTRON HF-ODS and HF-C4 column.
Shown below is analysis of positional isomers of cresol under water rich mobile phase condition with the ULTRON HF-Phenyl.
ULTRON HF-Phenyl Separation pattern of peptides comparison with other HF series columns
Shown below is comparison of peptides separation on the ULTRON HF series. The ULTRON HF-Phenyl and ULTRON HF-ODS columns are suitable for analysis of peptides. However, the peak profiles are different between the ULTRON HF-ODS and ULTRON HF-Phenyl.
ULTRON HF-Phenyl Fitting
| Fitting | Remarks |
|---|---|
| Standard specification (FEP sleeve) |
① 1/16” PEEK plug ② 1/16” O.D. x 0.35 mm I.D. x 40 mm FEP sleeve ③ 1/16” CTFE ferrule ④ 1/16” PEEK fitting ⑤ 1/32” PEEK column jacket ・ The above fittings corresponds to the VICI valve (ex. UltiMate 3000 RSLCnano system). ・ When installing the column to the system, tighten the column using a wrench (0.6 N.m as a guide). |
 |
|
| Pressure resistant specification (Inlet side: PEEK sleeve) |
① 1/16” PEEK plug ② 1/16” O.D. x 0.41 mm I.D. x 40 mm PEEK sleeve ③ 1/16” PEEK High pressure ferrule (Hybrid type of stainless steel and PEEK blended polymer) ④ 1/16” PEEK fitting ・ The above fittings corresponds to the VICI valve (ex. UltiMate 3000 RSLCnano system). ・ When tightening the ferrules use a wrench (1.4 N.m as a guide). ・ When installing the column to the system, tighten the column using a wrench (1.1 N.m as a guide). |
 |
The outer diameter of the capillary column is 0.38 mm. Please use suitable fittings (Sleeve, Ferrules, etc.) for your Nano Flow LC system. Normally, FEP sleeve fittings are shipped as a standard specification. Pressure resistant fittings (Inlet side: PEEK sleeve) are also available as option with additional cost.