Separation Methods Technologies, Incorporated (SMT, Inc.) is a surface chemistry research corporation. Their goal is to provide chromatographers with outstanding column packing materials and specialty columns for various separation chemistries ranging from analytical to process scale. Their primary focus is on the creation of well organized functional molecules on substrate surfaces for various functions, including chromatographic applications and materials engineering. SMT Inc. was founded in 1993. The company has its corporate headquarters in Newark, DE, with a worldwide distribution network.

Innovation: For over 25 years, SMT has repeatedly led the industry to new standards of measuring performance expected of capillary, UHPLC, HPLC and LPLC columns as well as the column packing materials.

Reproducibility: They are an Original Equipment Manufacturer (OEM), therefore, they have a complete control over quality and ensuring reproducibility of your chromatography methods.

Reliability: SMT’s policy has been to never change any product that has an existing customer base; however, when improvements are made, an entirely new SMT product line is introduced

Product Range: They have introduced over 100 stationary phases that have resulted in over 8,000 products for capillary, UHPLC, HPLC, MPLC and LPLC.

Separation Methods Technologies, Inc. (SMT) manufactures a full variety of capillary, UPLC, UHPLC, HPLC, LPLC, SFC, GPC, SEC and other chromatography columns and resins for all methods development, validation, and scale up needs.

Bulk Packing Material & Media

Normal Phase Columns

Bio LC Columns

Specialty Columns

Reversed-Phase Columns

Sample Preparation

Ion-Exchange Columns

GC Columns

SMT SAM Total Coverage packings are designed for usage from analytical to process scale purification. The primary purpose for analytical method developments in pharmaceutical and food industries is for subsequent process scale purification. The analytical steps are used to reduce the cost incurable in erroneous large-scale purification steps. SMT is a specialty column company, and as such, offers services that can enhance scale-up of your analytical methods development. Bulk samples are offered for your initial evaluation.

SMT manufactures high resolution and great efficiency columns for characterization of a full diversity of biomolecules. This includes the separation of proteins, peptides, monoclonal antibodies, carbohydrates, nucleic acids, and more. Their columns offer superb analytical characterization for biological samples by affinity, ion exchange, reversed phase, hydrophobic interaction, and size exclusion chromatography.

The use of high performance liquid chromatography (HPLC) for the separation and purification of organic compounds including pharmaceuticals, natural products, food additives, organic chemicals and biologicals, has increased dramatically in the past three decades. This increase is undoubtedly associated with the dramatic improvement in bonding chemistry.

In this mode of chromatography, the separation depends upon the exchange of ions between the mobile phase and the ionic sites of the packing (cationic or anionic). The stationary phase matrix has a functional group with a fixed ionic charge covalently attached to it . An exchangeable counterion from the mobile phase buffer preserves charge neutrality. The mobile phase usually contains a large number of counterions opposite in charge to the surface ionic group.

When chromatographic separation is done in a normal phase mode, the surface chemistry of the stationary phase has a polar characteristic. The mobile phase is generally nonpolar organic solvent (such as hexane or heptane). Because of the limited exibility in variation of the mobile phase polarity and functional stationary phase, separation in normal phase mode has not grown as much as reversed- phase mode. However, continuous advancement in surface modi cation has rejuvenated interest in normal phase chromatography. Furthermore, there are some separations that are achieved more conveniently using normal mode.

SMT has special interest in surface modification biotechnology and materials engineering. When separation is difficult with conventional bonded phases, SMT assists in method development and special column design for new applications. SMT specialty columns include special columns designed for reversed phase, normal phase, ion exchange chromatography, Gel Filtration/Size Exclusion Chromatography, Affinity Chromatography, Chiral and more. These columns are specially designed for companies that are interested in having competitive advantage in separation and surface modification.

Solid-Phase Extraction (SPE) is a sample preparation technique that is used to clean up and/or concentrate samples before analysis. Compared to liquid-liquid extraction, SPE is much faster, uses less solvent, and saves money. SPE provides clean extracts and high recoveries. SMT TotalSep™ SPE cartridges have been developed to meet some of the most dif culty challenges in sample puri cation methods: clean extracts and high recoveries.

SMT provides GC capillary columns and accessories for Gas chromatography. checkout our online catalog by clicking on the categories below, and discover one of the most complete range of stationary phase, columns and parts for your GC analysis.