MonoSpin ProA, MonoSpin ProG are immobilized with protein A or protein G onto a silica monolith offering rapid purification of antibodies. The silica is modified with a hydrophilic polymer and then immobilized with either Protein A or Protein G to prevent the adsorption of protein, resulting in rapid purification and high recovery or antibodies.
Antibody purification within 10 mins
Compare to conventional soft gels, using hard silica monolith as a support it can get a high-speed processing. Only 10 mins antibody can be purified by centrifuge.
MonoSpin ProA, 90 % recovery rate of IgG (maximum 0.4 mg).
MonoSpin ProG, 90 % recovery rate of IgG (maximum 0.3 mg).
MonoSpin ProG enables to recover and purify IgG3 which ProA cannot.
Relation between amount of IgG addiction and collected antibodies (MonoSpin ProA (left), ProG (right))
More than 90 % Recovery Rate realized with 100 µL eluent
MonoSpin ProA only requires 100 µL elution buffer, providing a recovery rate of at least 90 % IgG. On the other hand, other brands’ products requires 400 µL of elution buffer with a recovery rate of 70 % IgG.
Stable Recovery Rates and Reliable Reproducibility
Using MonoSpin 96-well Plates, antibody were purified from cultured CHO cells. It provided a stable recovery rates and reproducibility.
Result of antibody purification on 96-well Plates
The absorbance of the eluate was measured by MonoSpinProA 96WP purification using a sample of human polyclonal antibody added to the adsorption solution. Each well can be purified without variability.
Antibody additional volume 0.01 mg – 1.6 mg (100 µL repeat 2 times)
Wash: 1M NaCl-phosphoric buffer
(pH=7, 100 µL repeat 2 times)
Eluent : 0.1 M Citric acid buffer
(pH=3, 60 µL repeat 3 times)
Suction Conditions: 0.016 MPa (0.16 bar)
Possible to concentration of week sample with continuous flow
500 μL volume of 0.025 mg/mL of human IgG solution was applied to MonoSpin ProA spin column (ten consecutive times). Then, the elution of IgG concentration was measured with 100 μL elution buffer twice (E1 and E2). The first IgG elution (E1) was 50-fold concentration of the stock solution and showed 90 % recovery of IgG without the loss of IgG.